新しいページ 1

Publication List of Dr. Xuan, X.

2011

1. Luo, Y., Terkawi, M.A., Jia, H., Aboge, G.O., Goo, Y.K., Cao, S., Li, Y., Yu, L., Ooka, H., Kamyingkird, K., Masatani, T., Zhang, S., Nishikawa, Y., Igarashi, I., Xuan, X.: A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model. Exp. Parasitol. (in press)

2. Aung, K.M., Boldbaatar, D., Umemiya-Shirafuji, R., Liao, M., Xuan, X., Suzuki, H., Galay, R.L., Tanaka, T., Fujisaki, K.: Scavenger receptor mediates systemic RNA interference in ticks. PLoS One (in press)

3. Li, Y., Terkawi, M.A., Nishikawa, Y., Aboge, G.O., Luo, Y., Ooka, H., Goo, Y.K., Yu, L., Cao, S., Sun, Y., Yamagishi, J., Masatani, T., Yokoyama, N., Igarashi, I., Xuan, X.: Macrophages are critical for cross-protective immunity conferred by Babesia microti against Babesia rodhaini infection in mice. Infect. Immun. (in press)

4. Yamagishi, J., Watanabe, J., Goo, Y.K., Masatani, T., Suzuki, Y., Xuan, X.: Characterization of Toxoplasma gondii 5’ UTR with encyclopedic TSS information. J. Parasitol. (in press)

5. Tanaka, T., Maeda, H., Matsuo, T., Boldbattar, D., Umeniya-Shirafuji, R., Kume, A., Suzuki, H., Xuan, X., Tsuji, N., Fujisaki, K.: Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii. Peptides (in press)

6. Sandagdorj, N., Goo, Y.K., Badgar, B., Punsantsogvoo, M., Terkawia, M.A., Soma, T., Luo, Y., Li, Y., Cao, S., Yu, L., Kamyingkird, K., Aboge, G.O., Nishikawa, Y., Xuan, X.: Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay. Exp. Parasitol. (in press)

7. Yu, L., Zhang, S., Liang, W., Jin, C., Jia, L., Luo, Y., Li, Y., Cao, S., Yamagishi, J., Nishikawa, Y., Kawano, S., Fujisaki, K., Xuan, X.: Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR. J. Vet. Med. Sci. (in press)

8. Zhang, H., Lee, E.G., Yu, L., Kawano, S., Huang, P., Liao, M., Kawase, O., Zhang, G., Zhou, J., Fujisaki, K., Nishikawa, Y., Xuan, X.: Identification of the cross-reactive and species-specific antigens between Neospora caninum and Toxoplasma gondii tachyzoites by a proteomics approach. Parasitol. Res. (in press)

9. Yamagishi, J., Wakaguri, H., Sugano, S., Kawano, S., Fujisaki, K., Sugimoto, C., Watanabe, J., Suzuki, Y., Kimata, I., Xuan, X.: Construction and analysis of full-length cDNA library of Cryptosporidium parvum. Parasitol. Int. (in press)

10. Terkawi, M.A., Seuseu, F.J., Eko-Wibowo, P., Huyen, N.X., Minoda, Y., Aboulaila, M., Kawai, S., Yokoyama, N., Xuan, X., Iagarashi, I.: Secretion of a new spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes. Mol. Biochem. Parasitol. (in press)

11. Geriletu, Xu, R., Jia, H., Terkawi, M.A., Xuan, X., Zhang, H.: Immunogenicity of orally administrated recombinant Lactobacillus casei zhang expressing Cryptosporidium parvum surface adhesion protein P23 in mice. Curr. Microbiol. (in press)

12. Terkawi, M.A., Huyen, N.X., Shinuo, C., Inpankaew, T., Maklon, K., Aboulaila, M., Ueno, A., Goo, Y.K., Yokoyama, N., Jittapalapong, S., Xuan, X., Igarashi, I.: Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand. Vet. Parasitol. (in press)

13. Luo, Y., Jia, H., Terkawi, M.A., Goo, Y.K., Kawano, S., Ooka, H., Li, Y., Yu, L., Cao, S., Yamagishi, J., Fujisaki, K., Nishikawa, Y., Saito-Ito, A., Igarashi, I., Xuan, X.: Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test. Parasitol. Int. (in press)

14. Nishikawa, Y., Ibrahim, H.M., Kameyama, K., Shiga, I., Hiasa, J., Xuan, X.: Host cholesterol synthesis contributes to growth of intracellular Toxoplasma gondii in macrophages. J. Vet. Med. Sci. (in press)

15. Sivakumar, T., Khukhuu, A., Igarashi, I., Xuan, X., Guo, G., Huang, X., Yokoyama, N.: Phylogenetic analysis of Theileria orientalis in cattle bred in Fujian province, China. J. Protozool. Res. 21: 14-19, 2011.

16. Khukhuu, A., Lan, D.T., Long, P.T., Ueno, A., Li, Y., Luo, Y., Macedo, A.C., Matsumoto, K., Inokuma, H., Kawazu, S.I., Igarashi, I., Xuan, X., Yokoyama, N.: Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue Province, Vietnam. J. Vet. Med. Sci. (in press)

17. Terkawi, M.A., Huyen, N.X., Wibowo, P.E., Seuseu, F.J., Aboulaila, M., Ueno, A., Goo, Y.K., Yokoyama, N., Xuan, X., Igarashi, I.: Spherical body protein 4 is a new serological antigen for the global detection of Babesia bovis infection in cattle. Clin. Vaccine Immunol. 18: 337-342, 2011.

18. Kawase, O., Goo, Y.K., Jujo, H., Nishikawa, Y., Xuan, X.: Starfish, Asterias amurensis and Asterina pectinifera, as potential sources of Th1 immunity-stimulating adjuvants. J. Vet. Med. Sci. 73: 227-229, 2011.

19. Ooka, H., Terkawi, M.A., Goo, Y.K., Luo, Y., Li, Y., Yamagishi, J., Nishikawa, Y., Igarashi, I., Xuan, X.: Babesia microti: Molecular and antigenic characterizations of a novel 94-kilodalton protein (BmP94). Exp. Parasitol. 127: 287-293, 2011.

20. Tuda, J., Mongan, A., Tolba, M., Imada, M., Yamagishi, J., Xuan, X., Wakaguri, H., Sugano, S., Sugimoto, C., Suzuki, Y.: Full-parasites: Databases of full-length cDNAs of apicomplexa parasites, 2010 update. Nucleic Acids Res. 39: D625-631, 2011

2010

21. Zhang, G., Huang, X., Boldbaatar, D., Battur,B., Battsetseg, B., Zhang, H., Yu, L., Li, Y., Luo, Y., Cao, S., Goo, Y.K., Yamagishi, J., Zhou, J., Zhang, S., Suzuki, H., Igarashi, I., Mikami, T., Nishikawa, Y., Xuan, X.: Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. Vaccine 28: 7243-7247, 2010.

22. Yamagishi, J., Wakaguri, H., Ueno, A., Goo, Y.K., Tolba, M., Igarashi, M., Nishikawa, Y., Sugimoto, C., Sugano, S., Suzuki, Y., Watanabe, J., Xuan, X.: High-resolution characterization of Toxoplasma gondii transcriptome with a massive parallel sequencing method. DNA Res. 17: 233-243, 2010.

23. Aboge, G.O., Xuan, X.: Babesia gibsoni: Trends in application of molecular biology and biotechnology for diagnosis, vaccine development and drug targets validation of the parasite. Parasitol. Res. Trends 57-76, 2010.

24. Ibrahim, H.M., Xuan, X., Nishikawa, Y.: Toxoplasma gondii cyclophilin 18 regulates the proliferation and migration of murin macrophages and spleen cells. Clin. Vaccine Immunol. 17: 1322-1329, 2010.

25. Boldbaatar, D., Umemiya-Shirafuji, R., Liao, M., Tanaka, T., Xuan, X., Fujisaki, K.: Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development. J. Insect Physiol. 56: 1587-1598, 2010.

26. Nishikawa, Y., Zhang, H., Ibrahim, H.M., Yamada, K., Nagasawa, H., Xuan, X.: Roles of CD122(+) cells in resistance against Neospora caninum infection in a murine model. J. Vet. Med. Sci. 72: 1275-1282, 2010.

27. Aboge, G.O., Batbaatar, V., Goo, Y.K., Yamagishi, J., Nishikawa, Y., Sunaga, F., Namikawa, K., Igarashi, I., Fujisaki, K., Suzuki, H., Xuan, X.: Molecular characterization and expression of a 47- kDa merozoite surface protein of Babesia gibsoni for serodiagnosis by enzyme-linked immunosorbent assay. J. Protozool. Res. 20: 59-69, 2010.

28. Aboge, G.O., Jia, H., Terkawi, M., Goo, Y.K., Batbaatar, V., Nishikawa, Y., Sunaga, F., Namikawa, K., Igarashi, I., Fujisaki, K., Suzuki, 1., Xuan, X.: Precursors of methotrexate target dihydrofolate reductase-thymidylate synthase of Babesia gibsoni and inhibit parasite proliferation. J. Protozool. Res. 20: 70-81, 2010.

29. Goo, Y.K., Terkawi, M.A., Jia, H., Aboge, O., Ooka, H., Nelson, B., Kim, S., Sunaga, F., Namikawa, K., Igarashi, I., Nishikawa, Y., Xuan, X.: Artesunate, a potential drug for treatment of Babesia infection. Parasitol. Int. 59: 481-486, 2010.

30. Tanaka, T., Rahman, M.M., Battur, B., Boldbaatar, D., Liao, M., Umemiya-Shirafuji, R., Xuan, X., Fujisaki, K.: Parasiticidal activity of human alpha-defensin-5 against Toxoplasma gondii. In Vitro Cell Dev. Biol. Anim. 46: 560-565, 2010.

31. Iseki, H., Zhou, L., Kim, C., Inpankaew, T., Sununta, C., Yokoyama, N., Xuan, X., Jittapalapong, S., Igarashi, I.: Seroprevalence of Babesia infections of dairy cows in northern Thailand. Vet. Parasitol. 170: 193-196, 2010.

32. Herbas, M.S., Ueta, Y.Y., Ichikawa, C., Chiba, M., Ishibashi, K., Shichiri, M., Fukumoto, S., Yokoyama, N., Takeya, M., Xuan, X., Arai, H., Suzuki, H.: Alpha-tocopherol transfer protein disruption confers resistance to malaria infection in mice. Malar, J. 9: 101, 2010.

33. Tumurjav, B., Terkawi, M.A., Zhang, H., Zhang, G., Jia, H., Yamagishi, J., Nishikawa, Y., Igarashi, I., Sugimoto, C., Xuan, X.: Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1. Jpn. J. Vet. Res. 58: 111-119, 2010.

34. Hirano, S., Sato, G., Kobayashi, Y., Itou, T., Luo, R.T., Liu, Q., Jin, N.Y., Xuan, X., Sakai, T.: Analysis of Chinese rabies virus isolates from 2003-2007 based on P and M protein genes. Acta Virol. 54: 91-98, 2010.

35. Kawase, O., Nishikawa, Y., Bannai, H., Matsuo, T., Xuan, X.: Characterization of a novel thrombospondin-related protein in Toxoplasma gondii. Parasitol. Int. 59: 211-216, 2010.

36. Zhang, H., Nishikawa, Y., Yamagishi, J., Zhou, J., Kojima, N., Yokoyama, N., Xuan, X.: Neospora caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice. Exp. Parasitol. 125: 130-136, 2010.

37. Zhou, J., Liao, M., Gong, H., Xuan, X., Fujisaki, K.: Characterization of Hlcyst-3 as a member of cystetins from the tick Haemaphysalis longicornis. Exp. Appl. Acarol. 51: 327–333, 2010.

38. Jia, H., Nishikawa, Y., Luo, Y., Yamagishi, J., Sugimoto, C., Xuan, X.: Characterizaion of a leucine aminopeptidase from Toxoplasma gondii. Mol. Biochem. Parasitol. 170: 1-6, 2010.

39. Inpankaew, T., Pinyopanuwut, N., Chimno, W., Kengradomkit, C., Sununta, C., Zhang, G., Nishikawa, Y., Igarashi, I., Xuan, X., Jittapalapong, S.: Serodiagnosis of Toxoplasma gondii infection in dairy cows in Thailand. Transbound. Emerg. Dis. 57: 42-45, 2010.

40. Herbas, M.S., Okazaki, M., Terao, E., Xuan, X., Arai, H., Suzuki, H.: Alpha-tocopherol transfer protein inhibition is effective in the prevention of cerebral malaria in mice. Am. J. Clin. Nutr. 91: 200-2007, 2010.

2009

41. Hosono, H., Ito, S., Kono, H., Xuan, X.: Seroprevalence of Toxoplasma gondii in cats and pigs from T. T. Hue province in Vietnam. J. Vet. Epidemiol. 13: 100-106, 2009.

42. Goo, Y.K., Jia, H., Aboge, G.O., Terkawi, A.M., Yamagishi, J., Nishikawa, Y., Fujisaki, K., Xuan, X.: Identification of an immunodominant Babesia gibsoni 47-kDa antigen. J. Protozool. Res. 19: 16-20, 2009.

43. Herbas, H.M., Thekisoe, O.M., Inoue, N., Xuan, X., Arai, H., Suzuki, H.: The effect of alpha-tocopherol transfer protein gene disruption on Trypanosoma congolense infection in mice. Free Radic. Biol. Med. 47: 1408-1413, 2009.

44. Fukumoto, S., Tamaki, Y., Igarashi, I., Suzuki, H., Xuan, X.: Immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs. Exp. Parasitol. 123: 296-301, 2009.

45. Bannai, H., Nishikawa, Y., Ibrahim, H., Yamada, K., Kawase, O., Watanabe, J., Sugimoto, C., Xuan, X.: Overproduction of the pro-apoptotic molecule, programmed cell death 5, in Toxoplasma gondii leads to increased apoptosis of host macrophages. J. Vet. Med. Sci. 71: 1183-1189, 2009.

46. Ibrahim, H. M., Bannai, H., Xuan, X., Nishikawa, Y.: Toxoplasma gondii cyclophilin 18-mediated production of nitric oxide induces bradyzoite conversion in a CCR5-dependent manner. Infect. Immun. 77: 3686-3695, 2009.

47. Goo, Y.K., Jia, H., Terkawi, A.M., Aboge, G.O., Yamagishi, J., Nishikawa, Y., Fujisaki, K., Xuan, X.: Babesia gibsoni: Identification, expression, localization, and serological characterization of a 22-kDa protein. Exp. Parasitol. 123: 273-276, 2009.

48. Zhang, H., Thekisoe, O.M., Aboge, G.O., Kyan, H., Yamagishi, J., Inoue, N., Nishikawa, Y., Zakimi, S., Xuan, X.: Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method. Exp. Parasitol. 122: 47-50, 2009.

49. Hirose, Y., Matsuhira, S., Batanova, T.A., Goo, Y.K., Terkawi, M.A., Nishikawa, Y., Kitoh, K., Xuan, X., Takashima, Y.: Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins. J. Vet. Med. Sci. 71: 1133-1136, 2009.

50. Battsetseg, B., Boldbaatar, D., Battur, B., Xuan, X., Fujisaki, K.: Cloning and molecular characterization of tick kynurenin aminotransferase (HlKAT) from Haemaphysalis longicornis (Acari: Ixodidae). Parasitol. Res. 105: 669-679, 2009.

51. Terkawi, M.A., Amornthep, A., Ooka, H., Aboge, G., Jia, H., Goo, Y.K., Nelson, B., Yamagishi, J., Nishikawa, Y., Igarashi, I., Kawazu, S.I., Fujisaki, K., Xuan, X.: Molecular characterizations of three distinct Babesia gibsoni rhoptry-associated protein-1s. Parasitology 136: 1147–1160, 2009.

52. Jia, H., Terkawi, M.A., Aboge,G.O.,Goo, Y.K., Luo, Y., Li, Y., Nishikawa, Y., Igarashi, I., Sugimoto, C., Fujisaki, K., Xuan, X.: Characterization of a leucine aminopeptidase of Babesia gibsoni. Parasitology 136: 945-952, 2009.

53. Inpankaew, T., Jittapalapong, S., Phasuk, J., Pinyopanuwut, N., Chimnoi, W., Kengradomkit, C., Sunanta, C., Zhang, G., Aboge, G.O., Nishikawa, Y., Igarashi, I., Xuan, X.: Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23. Onderstepoort J. Vet. Res. 76: 161-165, 2009.

54. Nishikawa, Y., Zhang, H., Huang, P., Zhang, G., Xuan, X.: Effects of a transferring antibody against Neospora caninum infection in a murine model. Vet. Parasitol. 160: 60-65, 2009.

55. Nishikawa, Y., Zhang, H., Ikehara, Y., Kojima, N., Xuan, X., Yokoyama, N.: Immunization with oligomannose-coated liposome-entrapped dense granule protein 7 protects dams and offspring from Neospora caninum infection in mice. Clin Vaccine Immunol. 16: 792-797, 2009.

56. Ibrahim, H.M., Huang, P., Salem, T.A., Talaat, R.M., Nasr, M.I., Xuan, X., Nishikawa, Y.: Prevalence of Neospora caninum and Toxoplasma gondii antibodies in northern Egypt. Am. J. Trop. Med. Hyg. 80: 263-267, 2009.

57. Gong, H., Umemiya, R., Zhou, J., Liao, M., Zhang, H., Jia, H., Nishikawa, Y., Xuan, X., Fujisaki, K.: Blocking the secretion of saliva by silencing the HlYkt6 gene in the tick Haemaphysalis longicornis. Insect Biochem. Mol. Biol. 39: 372-381, 2009.

58. Jia, H., Aboge, G.O., Terkawi, M.A., Goo, Y.K., Nishikawa, Y., Kuriki, K., Lee, K.K., Jang, H.K., Kim, S., Fujisaki, K., Xuan, X.: Genetic diversity of two selected antigen loci in Babesia gibsoni Asian genotype obtained from Japan and Jeju island of South Korea. Vet. Parasitol. 162: 142-146, 2009.

59. Jia, H., Terkawi, M.A., Aboge,G.O.,Goo, Y.K., Ma, L., Zhou, J., Nishikawa, Y., Igarashi, I., Fujisaki, K., Xuan, X.: Identification of a secreted antigen 3 from Babesia gibsoni. Clin. Vaccine Immunol. 16: 944-948, 2009.

60. Terkawi, M.A., Aboge, G., Jia, H., Goo, Y.K., Ooka, H., Yamagishi, J., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S.I., Fujisaki, K., Xuan, X.: Molecular and immunological characterization of Babesia gibsoni and B. microti heat shock protein-70. Parasite Immunol. 31: 328-340, 2009.

61. Zhou, J., Liao, M., Ueda, M., Gong, H., Xuan, X., Fujisaki, K.: Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis. Vet. Parasitol. 160: 180-183, 2009.

62. Goo, Y.K., Jia, H., Aboge, G.O., Terkawi, M.A., Lee, E.G., Yamagishi, J., Nishikawa, Y., Jang, H.K., Sunaga, F., Namikawa, K., Fujisaki, K., Xuan, X.: Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis. Parasitol. Int. 58: 55-60, 2009.

2008

63. Tanaka, T., Xuan, X., Kojima, A., Igarashi, I., Fujisaki, K., Shimazaki, K.: Expression and characterization of bovine lactoperoxidase by recombinant vaccinia virus. Cytotechnology 58: 127-133, 2008.

64. Terkawi, M.A., Aboge, G.O., Jia, H., Goo, Y.K., Ooka, H., Yamagishi, J., Nishikawa, Y., Kawazu, S.I., Fujisaki, K., Xuan, X.: Identification of a novel Babesia gibsoni 27-kDa protein as a serodiagnostic antigen. J. Protozool. Res. 18: 48-56, 2008.

65. Buates, S., Xuan, X., Igarashi, M., Sugimoto, C., Inoue, N.: The influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection. J. Protozool. Res. 18: 79-95, 2008.

66. Matsuu, A., Yamasaki, M., Xuan, X., Ikadai, H., Hikasa, Y.: In vitro evaluation of the growth inhibitory activities of 15 drugs against Babesia gibsoni (Aomori strain). Vet. Parasitol. 157: 1-8, 2008.

67. Unno, A., Suzuki, K., Xuan, X., Nishikawa, Y., Kitoh, K., Takashima, Y.: Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow. Parasitol. Int. 57: 515-518, 2008.

68. Konishi, K., Sakata, Y., Miyazaki, N., Jia, H., Goo, Y.K., Xuan, X., Inokuma, H.: Epidemiological survey of Babesia gibsoni infection in dogs in Japan by enzyme-linked immunosorbent assay using B. gibsoni thrombospondin-related adhesive protein antigen. Vet. Parasitol. 155: 204-208, 2008.

69. Aboge, G., Jia, H., Terkawi, M., Goo, Y.K., Nishikawa, Y., Sunaga, F., Namikawa, K., Igarashi, I., Suzuki, H., Fujisaki, K., Xuan, X.: Cloning, expression and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and the parasite proliferation. Antimicrob. Agents Chemother. 52: 4072-4080, 2008.

70. Tsuji, N., Miyoshi, T., Battsetseg, B., Matsuo, T., Xuan, X., Fujisaki, K.: A cysteine protease is critical for Babesia spp transmission in Haemaphysalis ticks. PLoS Pathog. 4: e1000062, 2008.

71. Boldbaatar, D., Battsetseg, B., Matsuo, T., Hatta, T., Umemiya-Shirafuji, R., Xuan, X., Fujisaki, K.: Tick vitellogenin receptor reveals critical role in oocyte development and transovarial transmission of Babesia parasite. Biochem. Cell Biol. 86: 331-334, 2008.

72. Kubota, N., Sakata, Y., Miyazaki, N., Itamoto, K., Bannai, H., Nishikawa, Y., Xuan, X., Inokuma, H.: Serological survey of Neospora caninum infection among dogs in Japan through species-specific ELISA. J. Vet. Med. Sci. 70: 869-872, 2008.

73. Terkawi, A., Zhang, G., Jia, H., Aboge, G., Goo, Y.K., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S., Fujisaki, K., Xuan, X.: C3 contributes to the cross-protective immunity induced by Babesia gibsoni phosphoriboprotein P0 against a lethal B. rodhaini infection. Parasite Immunol. 30: 365-370, 2008.

74. Bannai, H., Nishikawa, Y., Matsuo, T., Kawase, O., Watanabe, J., Sugimoto, C., Xuan, X.: Programmed Cell Death 5 from Toxoplasma gondii: A secreted molecule that exerts a pro-apoptotic effect on host cells. Mol. Biochem. Parasitol. 159: 112-120, 2008.

75. Boldbaatar, Kilonzo, R.M., Battur, B., Umemiya, R., Min, L., Tanaka, T., Xuan, X., Fujisaki, k.: Identification of two forms of cyclophilin from the hard tick Haemaphysalis longicornis. Process Biochem. 43: 615-625, 2008.

76. Goo, Y.K., Jia, H., Aboge, G.O., Terkawi, M.A., Kuriki, K., Nakamura, C., Kumagai, A., Zhou, J., Lee, E.G., Nishikawa, Y., Igarashi, I., Fujisaki, K., Xuan, X.: Babesia gibsoni: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP. Exp. Parasitol. 118: 555-560, 2008.

77. Nishikawa, Y., Zhang, H., Ibrahim, H. M., Ui, F., Ogiso, A., Xuan, X.: Construction of Toxoplasma gondii bradyzoite expressing the green fluorescent protein. Parasitol. Int. 57: 219-222, 2008.

78. Dautu, G., Ueno, A., Miranda, A., Mwanyumba, S., Munyaka, B., Carmen, G., Kariya, T., Omata, Y., Saito, A., Xuan, X., Igarashi, M.: Toxoplasma gondii: Detection of MIC10 antigen in sera of experimentally infected mice. Exp. Parasitol. 118: 362-371, 2008.

79. Takashima, Y., Suzuki, K., Xuan. X., Nishikawa, Y., Unno, A., Kitoh, K.: Detection of the initial site of Toxoplasma gondii reactivation in brain tissue. Int. J. Parasitol. 38: 601-607, 2008.

80. Jia, H., Terkawi, M.A., Aboge, G.O., Goo, Y.K., Zhou, J., Lee, E.G., Nishikawa, Y., Igarashi, I., Fujisaki, K., Xuan, X.: Babesia gibsoni: Identification of an immunodominant, interspersed repeat antigen. Exp. Parasitol. 118: 146-149, 2008.

81. Liao, M., Boldbatar, D., Gong, H., Huang, P., Umemiya, R., Harnnoi, T., Zhou, J., Tanaka, T., Suzuki, H., Xuan, X., Fujisaki, K.: Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks. Insect. Biochem. Mol. Biol. 38: 285-295, 2008.

82. Kim, C.M., Blanco, L.B., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.: Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples. Vet. Parasitol. 151: 158-163, 2008.

83. Gong, H., Liao, M., Zhou, J., Hatta, T., Huang, P., Zhang, G., Kanuka, H., Nishikawa, Y., Xuan, X., Fujisaki, K.: Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis. Vet. Parasitol. 151: 268-278, 2008.

84. Kim, C.M., Blanco, L.B., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.: Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by Babesia bovis and Babesia bigemina. Am. J. Trop. Med. Hyg. 78: 117-121, 2008.

85. Cruz-Flores, M.J., Claveria, F.G., Verdida, R., Xuan, X., Igarashi, I.: First detection of Babesia gibsoni infection in Philippine stray dogs by immunochromatographic test (ICT). Vet. Arhiv 78: 149-157, 2008.

2007

86. Huang, P., Liao, M., Zhang, H., Lee, E.G., Nishikawa, Y., Xuan, X.: The dense granule protein NcGRA7, a new marker for the serodiagnosis of Neospora infection in aborting cows. Clin. Vaccine Immunol. 14: 1640-1643, 2007.

87. Kim, C., Iseki, H., Herbas, M.S., Yokoyama, N., Suzuki, H., Xuan, X., Fujisaki, K., Igarashi, I.: Development of Taqman-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina. Am. J. Trop. Med. Hyg. 77: 837-841, 2007.

88. Aboge, G.O., Jia, H., Terkawi, M.A., Goo, Y.K., Kuriki, K., Nishikawa, Y., Igarashi, I., Suzuki, H., Xuan, X.: A novel 57-kDa merozoite protein of Babesia gibsoni is a prospective antigen for diagnosis and serosurvey of canine babesiosis by enzyme-linked immunosorbent assay. Vet. Parasitol. 149: 85-94, 2007.

89. Gong, H., Zhou, J., Liao, M., Hatta, T., Harnnoi, T., Umemiya, R., Inoue, N., Xuan, X., Fujisaki, K.: Characterization of a carboxypeptidase inhibitor from the tick Haemaphysalis longicornis. J. Insect. Physiol. 53: 1079-1087, 2007.

90. Zhang, G., Huong, T., Battur, J., Zhou, J., Zhang, H., Liao, M., Kawase, O., Lee, E.G., Dautu, G., Igarashi, M., Nishikawa, Y., Xuan, X.: A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice. Parasitology 134: 1339-1346, 2007.

91. Aboge, G.O., Jia, H., Kuriki, K., Zhou, J., Nishikawa, Y., Igarashi, I., Fujisaki, K., Suzuki, H., Xuan, X.: Molecular characterization of a novel 32-kDa merozoite antigen of Babesia gibsoni with a better diagnostic performance by enzyme-linked immunosorbent assay. Parasitology 134: 1185-1194, 2007.

92. Kim, C., Alhassan, A., Verdida, R.A., Yokoyama, N., Xuan, X., Fujisaki, K., Kawazu, S.I., Igarashi, I.: Development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis. Vet. Parasitol. 148: 137-143, 2007.

93. Kawase, O., Nishikawa, Y., Zhang, H., Zhang, G., Bannai, H., Jin, S., Lee, E.G., Xuan, X.: Proteomic analysis of calcium-dependent secretion in Toxoplasma gondii. Proteomics 7: 3718-3725, 2007.

94. Terkawi, M.A., Jia, H., Gabriel, A., Goo, Y.K., Nishikawa, Y., Yokoyama, N., Igarashi, I., Fujisaki, K., Xuan, X.: A shared antigen among Babesia species: Ribosomal phosphoprotein P0 as a universal babesial vaccine candidate. Parasitol. Res. 102: 35-40, 2007.

95. Dautu, G., Munyaka, B., Carmen, G., Zhang, G., Omata, Y., Xuan, X., Igarashi, M.: Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BGA1 and evaluation of their immunogenic potential. Exp. Parasitol. 116: 273-282, 2007.

96. Tsuji, N., Badger, B., Damdinsuren, B., Miyoshi, T., Xuan, X., Oliver, J.H., Fujisaki, K.: A babesial vector tick defensin against Babesia parasites. Infect. Immun. 75: 3633-3640, 2007.

97. Zhou, J., Liao, M., Ueda, M., Gong, H., Xuan, X., Fujisaki, K.: Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Peptides 28: 1304-1310, 2007.

98. Nishikawa, Y., Kawase, O., Vielemeyer, O., Suzuki, H., Joiner, K.A., Xuan, X., Nagasawa, H.: Toxoplasma gondii infection induces apoptosis in noninfected macrophages: role of nitric oxide and other soluble factors. Parasite Immunol. 29: 375-385, 2007.

99. Liao, M., Hatta, T., Umemiya, R., Huang, P., Jia, H., Gong, H., Zhou, J., Nishikawa, Y., Xuan, X., Fujisaki, K.: Identification of three protein disulfide isomerase members from Haemaphysalis longicornis tick. Insect Biochem. Mol. Biol. 37: 641-654, 2007.

100. Zhang, H., Lee, E.G., Liao, M., Comaore, M., Zhang, G., Kawase, O., Fujisaki, K., Sugimoto, C., Nishikawa, Y., Xuan, X.: Identification of ribosomal phosphoprotein P0 of Neospora caninim as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis. Mol. Biochem. Parasitol. 153: 141-148, 2007.

101. Boldbaatar, D., Battsetseg, B., Hatta, T., Miyoshi, T., Tsuji, N., Xuan, X., Fujisaki, K.: Valosin-containing protein from the hard tick Haemaphysalis longicornis: effects of dsRNA-mediated HIVCP gene silencing. Biochem. Cell Bio. 85: 384-394, 2007.

102. Fujii, K., Kakumoto, C., Kobayashi, M., Saito, S., Kariya, T., Watanabe, Y., Xuan, X., Igarashi, I., Suzuki, M.: Seroepidemiology of Toxoplasma gondii and Neospora caninum in seals around Hokkaido, Japan. J. Vet. Med. Sci. 69: 393-398, 2007.

103. Liao, M., Zhou, J., Hatta, T., Umemiya, R., Miyoshi, T., Tsuji, N., Xuan, X., Fujisaki, K.: Molecular characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks. Vet. Parasitol. 146: 148-157, 2007.

104. Harnnoi, T., Sakaguchi, T., Nishikawa, Y., Xuan, X., Fujisaki, K.: Molecular characterization and comparative study of 6 salivary gland metalloproteases from the hard tick, Haemaphysalis longicornis. Comp. Biochem. Physiol. B Biochem. Mol. Bio. 147: 93-101, 2007.

105. Jia, H., Liao, M., Lee, E.G., Nishikawa, Y., Inokuma, H., Ikadai, H., Matsuu, A., Igarashi, I., Xuan, X.: Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs. Parasitol. Res. 100: 1381-1384, 2007.

106. Zhou, J., Jia, H., Nishikawa, Y., Fujisaki, K., Xuan, X.: Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen. Vet. Parasitol. 145: 16-20, 2007.

107. Terkawi, M.A., Jia, H., Zhou, J., Lee, E.G., Igarashi, I., Fujisaki, K., Nishikawa, Y., Xuan, X.: Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B. microti infection in mice. Vaccine 25: 2027-2035, 2007.

108. Hatta, T., Umemiya, R., Liao, M., Gong, H., Harnnoi, T., Tanaka, M., Miyoshi, T., Boldbaatar, D., Battsetseg, B., Zhou, J., Xuan, X., Tsuji, N., Taylor, D., Fujisaki, K.: RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis. Parasitol. Res. 100: 847-854, 2007.

109. Tanaka, M., Liao, M., Zhou, J., Nishikawa, Y., Xuan, X., Fujisaki, K.: Molecular cloning of two caspase-like genes from the hard tick Haemaphysalis longicornis. J. Vet. Med. Sci. 69: 85-90, 2007.

110. Zhang, H., Compaore, M., Lee, E.G., Liao, M., Zhang, G., Sugimoto, C., Nishikawa, Y, Xuan, X.: Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and anti-NcAMA1 antibody inhibit host cell invasion by both parasites. Mol. Biochem. Paarasitol. 151: 205-212, 2007.

111. Fukumoto, S., Tamaki, Y., Okamura, M., Bannai, H., Yokoyama, N., Suzuki, T., Igarashi, I., Suzuki, H., Xuan, X.: Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs. Vaccine 25: 1334-1341, 2007.

112. Chahan, B., Jian, Z., Miyahara, K., Tanabe, S., Xuan, X., Sato, Y., Moritomo, T., Nogami, S., Mikami, T., Maruyama, S., Inokuma, H.: Detection of DNA closely related to ‘Candidatus Rickettsia principis’ in Haemaphysalis danieli recovered from cattle in Xinjiang Uygur Autonomous Region area, China. Vet. Parasitol. 144: 184-187, 2007.

113. Battsetseg, B., Mastuo, T., Xuan, X., Boldbaatar, D., Chee, S. H., Umemiya, R., Sakaguchi, T., Hatta, T., Zhou, J., Verdida, A. R., Taylor, D., Fujisaki, K.: Babesia parasites develop and are transmitted by the non-vector soft tick Ornithodoros moubata (Acari: Argasidae). Parasitology 134: 1-8, 2007.

2006

114. Jia, H., Zhou, J., Ikadai, H., Matsuu, A., Suzuki, H., Igarashi, I., Fujisaki, K., Xuan, X.: Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its potential use in serodiagnosis. Am. J. Trop. Med. Hyg. 75: 843-850, 2006.

115. Miyama, T., Inokuma, H., Itamoto, K., Okuda, M., Verdida, R., Xuan, X.: Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50. J. Vet. Med. Sci. 68: 1371-1373, 2006.

116. Harnnoi, T., Watchabunsook, S., Sakaguchi, T., Xuan, X., Fujisaki, K.: Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects. J. Vet. Med. Sci. 68: 1289-1295, 2006.

117. Harnnoi, T., Sakaguchi, T., Xuan, X., Fujisaki, K.: Identification of genes encoding cement-like antigens expressed in the salivary glands of Haemaphysalis longicornis. J. Vet. Med. Sci. 68: 1155-1160, 2006.

118. Zhou, J., Gong, H., Zhou, Y., Xuan, X., Fujisaki, K.: Identification of a glycine-rich protein from the tick Rhipicephalus haemaphysaloides and evaluation of its vaccine potential against tick feeding. Parasitol. Res. 100: 77-84, 2006.

119. Zhou, J., Zhang, G., Verdida, R. A., Nishikawa, Y., Fujisaki, K., Xuan, X.: A 38-kDa protein from Babesia gibsoni and its antibody response in an experimentally infected dog. Vet. Parasitol. 141: 345-348, 2006.

120. Zhou, J., Yang, J., Zhang, G., Nishikawa, Y., Fujisaki, K., Xuan, X.: Babesia gibsoni: an apical membrane antigen-1 homologue and its antibody response in the infected dogs. Exp. Parasitol. 114: 329-333, 2006.

121. Zakimi, S., Kyan, H., Oshiro, M., Sugimoto, C., Xuan, X., Fujisaki, K.: Genetic characterization of GRA6 genes from Toxoplasma gondii from pigs in Okinawa, Japan. J. Vet. Med. Sci. 68: 1105-1107, 2006.

122. Khan, A., Bohme, U., Kelly, K. A., Adlem, E., Brooks, K., Simmonds, M., Mungall, K., Quail, M. A., Arrowsmith, C., Chillingworth, T., Churcher, C., Harris, D., Collins, M., Fosker, N., Fraser, A., Hance, Z., Jagels, K., Moule, S., Murphy, L., O’Neil, S., Rajandream, A., Saunders, D., Seeger, K., Whitehead, S., Mayr, T., Xuan, X., Watanabe, J., Suzuki, Y., Wakaguri, H., Sugano, S., Sugimoto, C., Paulsen, I., Mackey, A. J., Roos, D. S., Hall, N., Berriman, M., Barrell, B., Sibley, L. D., Ajioka, J. W.: Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii. Genome Res. 16: 1119-1125, 2006.

123. Hatta, T., Kazama, K., Miyoshi, T., Umemiya, R., Liao, M., Inoue, N., Xuan, X., Tsuji, N., Fujisaki, K.: Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis. Int. J. Parasitol. 36: 1123-1132, 2006.

124. Zhou, J., Ueda, M., Umemiya, R., Battsetseg, B., Boldbaatar, D., Xuan, X., Fujisaki, K.: A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity. Insect Biochem. Mol. Biol. 36: 527-535.

125. Inoue, M., Xuan, X., Fujisaki, K., Igarashi, I., Suzuki, H.: Role of type I/II scavenger receptors in malarial infection in C57BL/6J mice. Am. J. Trop. Hyg. 75: 178-181, 2006.

126. Suzuki, T., Ueta, Y., Inoue, N., Xuan, X., Saitoh, H., Suzuki, H.: Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense. Am. J. Trop. Med. Hyg. 74: 1020-1025.

127. Zhou, J., Zhang, G., Fukumoto, S., Yokoyama, N., Suzuki, H., Igarashi, I., Fujisaki, K., Lin, J., Xuan, X.: A Babesia gibsoni thrombospondin related anonymous protein (BgTRAP) is an immunodominant antigen involved in the erythrocyte invasion. Mol. Biochem. Parasitol. 148: 190-198, 2006.

128. Fukumoto, S., Sakaguchi, T., You, M., Xuan, X., Fujisaki, K.: Tick troponin I-like molecule is a potent inhibitor for angiogenesis. Microvasc. Res. 71: 218-221. 2006.

129. Huang, X., Xuan, X., Verdida, R., Zhang, S., Yokoyama, N., Xu, L., Igarashi, I.: An immunochromatographic test for simultaneous serodiagnosis of both Babesia equi and B. caballi infections in horses. Clin. Vaccine Immunol. 13: 553-555, 2006.

130. Huang, X., Xuan, X., Yokoyama, N., Katayama, Y., Anzai, T., Igarashi, I.: Evaluation enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infection. Vet. Parasitol. 140: 158-161, 2006.

131. Laio, M., Ma, L., Bannai, H., Lee, E.G., Zhang, H., Xuan, X., Fujisaki, K.: Identification of a protein disulfide isomerase of Neospora caninum in excretory-secretory products and its IgA binding and enzymatic activities. Vet. Parasitol. 139: 47-56, 2006.

132. Chahan, B., Zhang, S., Seo, J., Nakamura, C., Zhang, G., Bannai, H., Jian, Z., Inokuma, H., Tuchiya, K., Sato, Y., Kabeya, H., Maruyama, S., Mikami, T., Xuan, X.: Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in donkeys in Western Xinjiang, China. J. Vet. Med. Sci. 68: 753-755, 2006.

133. Zhou, J., Huang, B., Suzuki, H., Fujisaki, K., Igarashi, I., Xuan, X.: Isolation and identification of an actin from Babesia gibsoni. J. Parasitol. 92: 208-210, 2006.

134. Zhou, J., Min, L., Hatta, T., Tanaka, M., Xuan, X., Fujisaki, K.: Identification of a follistatin-related protein from the tick Haemaphysalis longicornis and its effect on tick oviposition. Gene 372: 191-198, 2006.

135. Boonchit, S., Alhassan, A., Chan, B., Xuan, X., Yokoyama, N., Ooshiro, M., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I.: Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay. Vet. Parasitol. 137: 28-35, 2006.

136. Assenga, S.P., You, M., Shy, C.H., Yamagishi, J., Sakaguchi, T., Zhou, J., Kibe, M.K., Xuan, X., Fujisaki, K.: The use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for thick control. Parasitol. Res. 98: 111-118, 2006.

137. Boldbaatar, D., Sikalizyo Sikasunge, C., Battsetseg, B., Xuan, X., Fujisaki, K.: Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis. Insect Biochem. Mol. Biol. 36: 25-36, 2006.

138. Bannai, H., Nishikawa, Y., Seo, J., Nakamura, C., Zhang, S., Kimata, I., Takashima, Y., Li, J., Igarashi, I., Xuan, X.: Development and evaluation of an enzyme-linked immunosorbent assay using recombinant P23 for the detection of antibodies to Cryptosporidium parvum in cattle. J. Protozool. Res. 16: 9-15, 2006.

139. Hara, O., Min, L., Baticados, W., Bannai, H., Zhang, G., Zhang, S., Lee, E.G., Nishikawa, Y., Claveria, F., Igarashi, M., Nagasawa, H., Xuan, X.: Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis. J. Protozool. Res. 16: 34-41, 2006.

2005

140. Boldbaatar, D., Xuan, X., Battsetseg, B., Igarashi, I., Battur, B., Batsukh, Z., Bayambaa, B., Fujisaki, K.: Epidemiological study of equine piroplasmosis in Mongolia. Vet. Parasitol. 127: 35-38, 2005.

141. Gaturaga, I., Chahan, B., Xuan, X., Huang, H., Liao, M., Fukumoto, S., Hirata, H., Nishikawa, Y., Takashima, Y., Suzuki, H., Fujisaki, K., Sugimoto, C.: Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli. J. Parasitol. 91: 191-192, 2005.

142. Hirata, H., Yokoyama, N., Xuan, X., Fujisaki, K., Suzuki, N., Igarashi, I.: Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis. Clin. Diagn. Lab. Immunol. 12: 334-338, 2005.

143. Baticados, W. N., Witola, W. H., Inoue, N., Kim, J. Y., Kuboki, N., Xuan, X., Yokoyama, N, Sugimoto, C.: Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated. J. Vet. Med. Sci. 67: 157-64, 2005.

144. Fukumoto, S., Suzuki, H., Xuan, X.: Immunization with recombinant surface antigen P50 of Babesia gibsoni expressed in insect cells induced parasite growth inhibition in dogs. Clin. Diagn. Lab. Immunol. 12: 557-559, 2005.

145. Shirafuji, H., Xuan, X., Kimata, I., Takashima, Y., Shinya, F., Otsuka, H., Nagasawa, H., Suzuki, H.: Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects. J. Parasitol. 91: 476-479, 2005.

146. Liao, M., Xuan, X., Shirafuji, H., Fukumoto, S., Hirata, H., Suzuki, H., Fujisaki, K.: Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii. Parasitol. 130: 481-488, 2005.

147. Lee, D.S., Yanagimoto Ueta, Y., Xuan, X., Igarashi, I., Fujisaki, K., Sugimoto, C., Toyoda, Y., Suzuki, H.: Expression patterns of the implantation-associated genes in the uterus during the estrous cycle in mice. J. Reprod. Dev. 51: 787-798, 2005.

148. Miyama, T., Sakata, Y., Shimada, Y., Ogino, S., Watanabe, M., Itamoto, K., Okuda, M., Verdida, R. A., Xuan, X., Nagasawa, H., Inokuma, H.: Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan. J. Vet. Med. Sci. 67: 467-471, 2005.

149. Hori, T., Yamsaard, T., Yanagimoto Ueta, Y., Harakawa, S., Kaneko, E., Miyamoto, A., Xuan, X., Toyoda, Y., Suzuki, H.: Exposure of C57BL/6J male mice to an electric field improves copulation rates with superovulated females. J. Reprod. Dev. 51: 393-397, 2005.

150. Liao, M., Xuan, X., Zhang, S., Zhang, G., Huang, X., Igarashi, I., Fujisakia, K.: Development of a rapid immunochromatographic test with recombinant NcSAG1 for detection of Neospora caninum infection in cattle. Clin. Diagn. Lab. Immunol. 12: 885-887, 2005.

151. Fukumoto, S., Suzuki, H., Igarashi, I., Xuan, X.: Fatal experimental transplacental Babesia gibsoni infections in dogs. Int. J. Parasitol. 35:1031-1035, 2005.

152. Verdida, R. A., Xuan, X., Fukumoto, S., Huang, X., Zhou, J., Igarashi, I., Claveria, F. G., Nagasawa, H.: Development of a practical immunochromatographic test with recombinant P50 for the diagnosis of Babesia gibsoni infection in dogs. Parasitol. 131: 769-774, 2005.

153. Chahan, B., Jian, Z., Xuan, X., Sato, Y., Kabeya, H., Tuchiya, K., Itamoto, K., Okuda, M., Mikami, T., Maruyama, S., Inokuma, H.: Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur Autonomous Region area, China. Vet. Parasitol. 134: 273-278, 2005.

2004

154. Huang, X., Xuan, X., Hirata, H., Yokoyama, N., Xu, L., Suzuki, N., Igarashi, I.: A rapid immunochromatographic test with recombinant SAG2 for the detection of antibodies against Toxoplasma gondii in cats. J. Clin. Microbiol. 42: 351-353, 2004.

155. Huang, X., Xuan, X., Xu, L., Zhang, S., Yokoyama, N., Suzuki, N., Igarashi, I.: Development and evaluation of an immunochromatographic test for the rapid detection of Babesia equi infection in horses. J. Clin. Microbiol. 42: 359-361, 2004.

156. Kaneko, Y., Takashima, Y., Xuan, X., Igarashi, I., Nagasawa, H., Mikami, T., Otsuka, H.: Natural IgM antibodies in sera from various animals but not the cat kill Toxoplasma gondii by activating complement classical pathway. Parasitol. 128: 123-129, 2004.

157. Fukumoto, S., Xuan, X., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.: Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in canine red blood cell-substituted severe combined immunodeficiency mice. Infect. Immun. 72: 1795-1798, 2004.

158. Kumar, S., Yokoyama, N., Kim, J.Y., Huang, X., Inoue, N., Xuan, X., Igarashi, I., Sugimoto, C.: Expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton. Mol. Biochem. Parasitol. 133: 221-227, 2004.

159. Boonchit, S., Xuan, X., Yokoyama, N., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I.: An improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for the detection of specific antibodies. J. Clin. Microbiol. 42: 1601-1604, 2004.

160. Fukumoto, S., Sekine, Y., Xuan, X., Igarashi, I., Sugimoto, C., Nagasawa, H., Fujisaki, K., Mikami, T., Suzuki, H.: Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coli. J. Parasitol. 90: 387-391, 2004.

161. Matsuo, T., Okoda, Y., Badgar, B., Inoue, N., Xuan, X., Taylor, D., Fujisaki, K.: Fate of GFP-expressing Escherichia coli in the midgut and response to ingestion in a tick, Ornithodoros moubata (Acari: Argasidae). Exp. Parasitol. 108: 67-73, 2004.

162. Tamaki, Y., Hirata, H., Takabatake, N., Bork, S., Yokoyama, N., Xuan, X., Fujisaki, K., Igarashi, I.: Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 11: 211-215, 2004.

163. Verdida, R. A., Hara, O. A., Xuan, X., Fukumoto, S., Igarashi, I., Zhang, S., Dong, J., Inokuma, H., Kabeya, H., Sato, Y., Moritomom T., Maruyama, S., Claveria F., Nagasawa, H.: Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50. J. Vet. Med. Sci. 66: 1517-1521, 2004.

2003

164. Yokoyama, N., Bork, S., Nishisaka, M., Hirata, H., Matsuo, T., Inoue, N., Xuan, X., Suzuki, H., Sugimoto, C., Igarashi, I.: Roles of the Maltese cross form in the development of parasitemia and protection against Babesia microti infection in mice. Infect. Immun. 71: 411-417, 2003.

165. Nagai, A., Yokoyama, N., Matsuo, T., Bork, S., Hirata, H., Xuan, X., Zhu, Y., Claveria, F.G., Fujisaki, K., Igarashi, I.: Growth-inhibitory effects of artesunate, pyrimethamine, and pamaquine against Babesia equi and Babesia caballi in vitro cultures. Antimicrob. Agents Chemother. 47: 800-803, 2003.

166. Hirata, H., Xuan, X., Yokoyama, N., Nishikawa, Y., Fujisaki, K., Suzuki, N., Igarashi, I.: Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis. J. Clin. Microbiol. 41: 547-551, 2003.

167. Maruyama, S., Kabeya, H., Nakao, R., Tanaka, S., Sakai, T., Xuan, X., Katsube, Mikami, T.: Seroprevalence of Bartonella henselae, Toxoplasma gondii, FIV and FeLV infections in domestic cats in Japan. Microbiol. Immunol. 47: 147-153, 2003.

168. Tanaka, T., Nakatani, S., Xuan, X., Kumura, H., Igarashi, I., Shimazaki, K.: Antiviral activity of lactoferrin against canine herpesvirus. Antiviral Res. 60:193-199, 2003.

169. Huang, X., Xuan, X., Yokoyama, N., Xu, L., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Igarashi, I. High-level expression and purification of a truncated merozote antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis. J. Clin. Microbiol. 41: 1147-1151, 2003.

170. Takashima, Y., Xuan, X., Kimata, I., Iseki, M., Kodama, Y., Nagane, N., Nagasawa, H., Matsumoto, Y., Mikami, T., Otsuka, H.: Recombinant bovine herpesvirus-1 (BHV-1) expressing P23 protein of Cryptosporidium parvum induces neutralizing antibodies in rabbits. J. Parasitol. 89: 276-282, 2003.

171. Makala, L., Reyes, J.C., Nishikawa, Y., Tsushima, Y., Xuan, X., Huang, X., Battsetseg, B., Matsuo, T., Nagasawa, H.: Phenotype and function of murine discrete Peye’s patch macrophage derived- dendric cells. J. Vet. Med. Sci. 65: 491-499, 2003.

172. Fukumoto, S., Xuan, X., Kadota, K., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.: High-level expression of truncated major surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity. Clin. Diagn. Lab. Immunol. 10: 596-601, 2003.

173. Bayin, C., Gaturaga, I., Huang, X., Min, L., Fukumoto, S., Hirata, H., Nishikawa, Y., Suzuki, H., Sugimoto, C., Igarashi, I., Mikami, T., Xuan, X.: Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. Vet. Parasitol. 118: 177-185, 2003.

174. Fujii, K., Yokoyama, N., Takabatake, N., Suzuki, H., Xuan, X., Igarashi, I.: Transient expression of a green fluorescent protein gene in Babesia bovis. J. Protozool. Res. 13: 10-14, 2003.

175. Takashima, Y., Xuan, X., Hongo, H., Nagasawa, Y., Matsumoto, Y., Otsuka, H.: Immunogenic property of a recombinant vaccinia virus expressing P23 of Cryptosporidium parvum. J. Protozool. Res. 13: 15-22, 2003.

176. Tsushima, Y., Karanis, P., Kamada, T., Makala, L., Xuan, X., Tohya, Y., Akashi, H., Nagasawa, H.: Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method. J. Vet. Med. Sci. 65: 121-123, 2003.

177. Tsushima, Y., Karanis, P., Kamada, T., Xuan, X., Makala, L., Tohya, Y., Akashi, H., Nagasawa, H.: Viability and infectivity of Cryptosporidium parvum oocysts detected in river water in Hokkaido, Japan. J. Vet. Med. Sci. 65: 585-589, 2003.

178. Makala, L., Reyes, J.C., Nishikawa, Y., Tsushima, Y., Xuan, X., Huang, X., Nagasawa, H.: A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice. J. Vet. Med. Sci. 65: 591-597, 2003.

179. Xu, Y., Zhang, S., Huang, X., Bayin, C., Xuan, X., Igarashi, I., Fuzisaki, K., Kabeya, H., Maruyama, S., Mikami, T.: Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China. J. Vet. Med. Sci. 65: 1015-1017, 2003.

180. You, M., Xuan, X., Tsuji, N., Kamio, T., Taylor, D., Suzuki, N., Fujisaki, K. Identification and molecular characterization of a chitinase from the hard tick Haemaphysalis longicornis. J. Biol. Chem. 278: 8556-8563, 2003.

181. Fukumoto, S., Xuan, X., Inoue, N., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.: Molecular characterization of a gene encoding 29 kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. Mol. Biochem. Parasitol. 131: 129-139, 2003.

182. Tanaka, T., Abe, Y., Kim, W.S., Xuan, X., Nagasawa, H., Igarashi, I., Kumura, H., Shimazaki, K.: The detection of bovine lactoferrin binding protein on Toxoplasma gondii. J. Vet. Med. Sci. 65:1377-1380, 2003.

183. Nishikawa, Y., Xuan, X., Makala, L., Vielemeyer, O., Joiner, K.A., and Nagasawa, H.: Characterization of Toxoplasma gondii engineered to express mouse interferon-gamma. Int. J. Parasitol. 33: 1525-1535, 2003.

2002

184. Nishikawa, Y., Kimura, M., Xuan, X., Makala, L., Nagasawa, H., Mikami, T., Otsuka, H.: Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. Virus Res. 87:1-9, 2002.

185. Mishima, M., Xuan, X., Yokoyama, N., Igarashi, I., Fujisaki, K., Nagasawa, H., Mikami, T.: Recombinant feline herpesvirus type 1 expressing Toxoplasma gondii ROP2 antigen inducible protective immunity in cats. Parasitol. Res. 88:144-149, 2002.

186. Hirata, H., Ikadai, H., Yokoyama, N., Xuan, X., Fujisaki, K., Suzuki, N., Mikami, T., Igarashi, I.: Cloning of a truncated Babesia equi gene encoding an 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay. J. Clin. Microbiol. 40:1470-1474, 2002.

187. Ikadai, H., Nagai, A., Xuan, X., Igarashi, I., Tsugihiko, K., Tsuji, N., Oyamada, T., Suzuki, N., Fujisaki, K.: Seroepidemiologic studies on Babesia caballi and Babesia equi infection in Japan. J. Vet. Med. Sci. 64:325-328, 2002.

188. Fukumoto, S., Sekine, Y., Kimbita, E., Huang, X., Xuan, X., Inoue, N., Yokoyama, N., Igarashi, I., Fujisaki, K., Sugimoto, C., Nagasawa, H., Mikami, T., Suzuki, H.: Cloning and expression an antigen of Babesia gibsoni in Escherichia coli and its use for the immunodiagnosis of canine babesiosis. J. Protozool. Res. 12: 16-23, 2002.

189. Huang, X., Xuan, X., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Mikami, T., Igarashi, I.: Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 9:1343-1347, 2002.

190. Battsetseg, B., Lucero, S., Xuan, X., Claveria, F., Byambaa, B., Battur, B., Boldbaatar, D., Batsukh, Z., Khaliunaa, T., Battsetseg, G., Igarashi, I., Nagasawa, H., Fujisaki, K.: Detection of equine Babesia spp. Gene fragments in Dermacentor nuttalli olenev 1929 infesting Mongolian horses, and their amplification in egg and larval progenies. J. Vet. Med. Sci. 64:727-730, 2002.

191. Huang, X., Xuan, X., Kimbita, E.N., Battur, B., Miyazawa, T., Fukumoto, S., Mishima, M., Makala, L.H., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Mikami, T., Igarashi, I.: Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats. J. Parasitol. 88:804-807, 2002.

192. Battsetseg, B., Lucero, S., Xuan, X., Claveria, F., Inoue, N., Alhassan, A., Kanno, T., Igarashi, I., Nagasawa, H., Mikami, T., Fujisaki, K.: Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction Vet. Parasitol. 107:351, 2002.

193. Xuan, X., Chahan, B., Huang, X., Yokoyama, N., Makala, L., Igarashi, I., Fujisaki, K., Maruyama, S., Sakai, T., Mikami, T.: Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens. Vet. Parasitol. 108:179-181, 2002.

194. Makala, L.H., Nishikawa, Y., Mishima, M., Inoue, N., Xuan, X., Suzuki, H., Fujisaki, K., Mikami, T., Nagasawa, H.: Phenotype and function of murine peritoneal cavity macrophage derived-dendritic cells. J. Vet. Med. Sci. 64:813-820, 2002.

195. Boonchit, S., Xuan. X., Yokoyama, N., Goff, W.L., Wagner, G., Igarashi, I.: Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle. J. Clin. Microbiol. 40:3771-3775, 2002.

196. Nishikawa, Y., Tragoolpua, K., Makala, L., Xuan, X., Nagasawa, H.: Neospora caninum NcSRS2 is a transmembrane protein that contains a glycosylphosphatidylinositol anchor in insect cells. Vet. Parasitol. 109:191-201, 2002.

2001

197. Nishikawa, Y., Inoue, N., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., Mikami, T.: Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection. Vaccine 19:1381-1390, 2001.

198. Mishima, M., Xuan, X., Nishikawa, Y., Makala, L., Yokoyama, N., Nagasawa, H., Mikami, T.: Construction of recombinan feline herpesvirus type 1 expressing Toxoplasma gondii surface antigen 1. Mol. Biochem. Parasitol. 117:103-106, 2001.

199. Xuan, X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I., Nagasawa, H., Fujisai, K., Toyoda, Y., Suzuki, N., Mikami, T.: Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. J. Clin. Microbiol. 39:705-709, 2001.

200. Nishikawa, Y., Iwata, A., Katsumata, A., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., Mikami, T.: Expression of canine interferon-γ by a recombinant vaccinia virus and its antiviral effect. Virus Res. 75:113-121, 2001.

201. Nishikawa, Y., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., Mikami, T.: Prevention of vertical transmission of Neospora caninum in BALB/c mice by recombinant vaccinia virus carrying NcSRS2 gene. Vaccine 19:1710-1716, 2001.

202. Makala, L.H., Nishikawa, Y., Kamada, T., Xuan, X., Nagasawa, H.: Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells. Pathobiology 69:104-112, 2001.

203. Tsushima, Y., Karanis, P., Kamada, T., Nagasawa, H., Xuan, X., Igarashi, I., Fujisaki, K., Takahashi, E., Mikami, T.: Detection of Cryptosporidium parvum oocysts in enviromental water in Hokkaido, Japan. J. Vet. Med. Sci. 63:233-236, 2001.

204. Xuan, X., Igarashi, I., Tanaka, T., Fukumoto, S., Nagasawa, H., Fujisaki, K., Mikami, T.: Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1. Clin. Diagn. Lab. Immunol. 8:645-646, 2001.

205. Badgar, B., Xuan, X., Rpdriguez, J. L., Badarch, B., Damdinsuren, B., Banzragch, B., gonchigoo, B., zayat, B., Ikadai, H., Igarashi, I., Nagasawa, H., Mikami, T., Fujisaki, K.: Detection of Babesia caballi and Babesia equi in Dermacentor nuttali adult ticks. Int. J. Parasitol. 31:384-386, 2001.

206. Mishima, M., Xuan, X., Shiota, A., Omata, Y., Fujisaki, K., Nagasawa, H., Mikami, T.: Modified protection against Toxoplasma gondii lethal infection and brain cyst formation by vaccination with SAG2 and SRS1. J. Vet. Med. Sci. 63:433-438, 2001.

207. Koyama, T., Shimada, S., Ohsawa, T., Omata, Y., Xuan, X., Inoue, N., Mikami, T., Saito, A.: A 14-3-3 protein homologue is expressed in feline enteroepithelia-stages of Toxoplasma gondii. Vet. Parasitol. 96:65-74, 2001.

208. Fukumoto, S., Xuan, X., Nishikawa, Y., Inoue, N., Igarashi, I., Nagasawa, H., Fujisaki, K., Mikami, T.: Identification and expression of a 50-kilodalton surface antigen of Babesia gibsoni and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. J. Clin. Microbiol. 39:2603-2609, 2001.

209. Boldbaatar, D., Xuan, X., Kimbita, E., Huang, X., Igarashi, I., Byambaa, B., Battsetseg, B., Battur, B., Battsetseg, G., Batsukh, Z., Nagasawa, H., Fujisaki, K., Mikami, T.: Detection of antibodies to Hypoderma lineatum in cattle by Western blotting with recombinant hypodermin C antigen. Vet. Parasitol. 99:147-154, 2001.

210. Fukumoto, S., Xuan, X., Shigeno, S., Kimbita, E., Igarashi, I., Nagasawa, H., Fujisaki, K., and Mikami, T.: Development of a polymerase chain reaction method for diagnosing Babesia gibsoni infection in dogs. J. Vet. Med. Sci. 63:977-981, 2001.

211. Xuan, X., Nagai, A., Battsetseg, B., Fukumoto, S., Makala, L.H., Inoue, N., Igarashi, I., Mikami, T., Fujisaki, K.: Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens. J. Vet. Med. Sci. 63:1159-1160, 2001.

212. Nishikawa, Y., Kousaka, Y., Tragoolpua, K., Xuan, X., Makala, L., Fujisaki, K., Mikami, T., Nagasawa, H.: Characterization of Neospora caninum surface protein NcSRS2 based on baculovirus expression system and its application for serodiagnosis of Neospora infection. J. Clin. Microbiol. 39:3987-3991, 2001.

213. Kimbita, E.N., Xuan, X., Huang, X., Miyazawa, T., Fukumoto, S., Mishima, M., Suzuki, H., Sugimoto, C., Nagasawa, H., Fujisaki, K., Suzuki, N., Mikami, T., Igarashi, I.: Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1. Vet. Parasitol. 102:35-44, 2001.

214. You, M., Xuan, X., Tsuji, N., Kamio, T., Igarashi, I., Nagasawa, H., Mikami, T., Fujisaki, K.: Molecular characterization of a troponin I-like protein from the hard tick Haemaphsalis longicornis. Insect Biochem. Mol. Biol. 32:67-73, 2001.

215. Nishisaka, M., Yokoyama, N., Xuan, X., Inoue, N., Nagasawa, H., Fujisaki, K., Mikami, T., Igarashi, I.: Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperonin containing T-complex protein 1. Int. J. Parasitol. 31:1673-1679, 2001.

216. Makala, L.H., Nishikawa, Y., Kamada, T., Suzuki, H., Xuan, X., Igarashi, I., Nagasawa, H.: comparison of the accessory activity of murine peritoneal cavity macrophage derived dendric cells and peritoneal cavity macrophages in a mixed lymphocyte reaction. J. Vet. Med. Sci. 63:1271-1277, 2001.

2000

217. Nishikawa, Y., Iwata, A., Xuan, X., Nagasawa, H., Fujisaki, K., Otsuka, H., Mikami, T.: Expression of canine interferon-β by a recombinant vaccinia virus. FEBS Letter 466:179-182, 2000.

218. Nishikawa, Y., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., Mikami, T.: Monoclonal antibody inhibition of Neospora caninum tachyzoite invasion into host cells. Int. J. Parasitol. 30: 51-58, 2000.

219. Ikadai, H., Kabamoto, S., Xuan, X., Igarashi, I., Nagasawa, H., Fujisaki, K., Suzuki, N., Mikami, T.: Protein analysis of Babesia caballi merozoites by two-dimentional polyacrylamide gel electrophoresis and western blotting. J. Vet. Med. Sci. 62:323-327, 2000.

220. Seng, S., Yokoyama, M., Suzuki, R., Maki, Y., Kato, M., Lim, B., Zayantiin, B., Inoue, N., Xuan, X., Igarashi, I., Fujisaki, K., Mikami, T., Suzuki, N., Toyoda, Y.: Expression of SAG-1 of Toxoplasma gondii in transgenic mice. Parasitol. Res. 86:263-269, 2000.

221. Ikadai, H., Osorio, C. R., Xuan, X., Igarashi, I., Kanemura, T., Nagasawa, H., Fujisaki, K., Suzuki, N., Mikami, T.: Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozote rhoptry protein. Int. J. Parasitol. 30: 633-635, 2000.

222. Igarashi, I., Asaba, U., Xuan, X., Omata, Y., Saito, A., Nagasawa, H., Fujisaki, K., Suzuki, N., Iwakura, Y., Mikami, T.: Immunization with recombinant surface antigens p26 with Freun’s adjuvants against Babesia rodhaini infection. J. Vet. Med. Sci. 62:717-723, 2000.

223. Mishima, M., Xuan, X., Takeiri, A., Makala, L., Iagarashi, I., Fujisaki, K., Nagasawa, H., Mikami, T.: Construction of recombinant feline herpesvirus type 1 expressing Toxoplasma gondii SRS2. J. Protozool. Res. 10: 166-172, 2000.

224. Ikeda, Y., Shibata, I., Xuan, X., Matsumoto, Y., Otsuka, H.: Immunogenic properties of a bovine herpesvirus-1 (BHV-1) recombinant expressing major psedorabies virus (PrV) glycoproteins in combination. J. Vet. Med. Sci. 62: 849-859, 2000.

225. Fukumoto, S., Xuan, X., Igarashi, I., Zhang, S., Mugisha, J., Ogata, T., Nagasawa, H., Fujisaki, K., Suzuki, N., Mikami, T.: Morphological changes of Babesia gibsoni grown in canine red blood cell-substituted servere combined immune deficiency mice. J. Parasitol. 86:956-959, 2000.

226. Koyama, T., Shimada, S., Ohsawa, T., Omata, Y., Xuan, X., Inoue, N., Mikami, T., Saito, A.: Antigens expressed in feline enteroepithelial-stages parasites of Toxoplasma gondii. J. Vet. Med. Sci. 62:1089-1092, 2000.

227. Takashima, Y., Xuan, X., Nagasawa, H., Matsumoto, Y., Igarashi, I., Fujisaki, K., Mikami, T., Otsuka, H.: Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, P23. J. Protozool. Res. 10:173-179, 2000.

228. Nishikawa, Y., Kousaka, Y., Fukumoto, S., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., Mikami, T.: Delivery of Neospora caninum surface protein, NcSRS2 (Nc-p43), to mouse using recombinant vaccinia virus. Parasitol. Res. 86:934-939, 2000.

229. Nishikawa, Y., Ikeda, H., Fukumoto, S., Xuan, X., Nagasawa, H., Otsuka, H., Mikami, T.: Immunisation of dogs with a canine herpesvirus vector expressing Neospora caninum surface protein, NcSRS2. Int. J. Parasitol. 30:1167-1171, 2000.

1999

230. Takashima, Y., Tamura, H., Xuan, X., Otsuka, H.: Identification of the US3 gene product of BHV-1 as a protein kinase and characterization of BHV-1 mutants of the US3 gene. Virus Res. 59:23-34, 1999.

231. Seng, S., Maki, Y., Yokoyama, M., Suzuki, R., Kato, M., Bray, R. L., Xuan, X., Igarashi, I., Nagasawa, H., Fujisaki, K., Mikami, T., Suzuki, N. Toyoda, Y.: Non-invasive method of identification of SAG-1 transgenic mice by PCR analysis of oral wash cells. J. Protozool. Res. 9:10-16, 1999.

232. Xuan, X., Zhang, S., Kamio, T., Tsushima, Y., Nishikawa, Y., Otsuka, H., Igarashi, I., Nagasawa, H., Fujisaki, K., Mikami, T.: Characterization of P15 antigen of Cryptosporidium parvum expressed by a recombinant vaccinia virus. J. Protozool. Res. 9:32-40, 1999.

233. Nishikawa, Y., Xuan, X., Otsuka, H.: Biosynthesis and interaction of glycoprotein E and I of canine herpesvirus. Virus Res. 61:29-41, 1999.

234. Murata, T., Takashima, Y., Xuan, X., Otsuka, H.: Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells. Virus Res. 61:65-73, 1999.

235. Murata, T., Xuan, X., Otsuka, H.: Characterization of promoter integrated in the genome of bovine herpesvirus-1 (BHV-1). J. Vet. Med. Sci. 61:453-457, 1999.

236. Takashima, Y., Xuan, X., Matsumoto, Y., Onuma, M., Otsuka, H.: Antibodies produced by mice immunized with recombinant vaccinia virus expressing two different types of a major Theileria sergenti surface antigen (p32) react with the native surface antigen. Vet. Parasitol. 84:65-73, 1999.

237. Seng, S., Nagasawa, H., Maki, Y., Yokoyama, M., Inoue, N., Xuan,X., Igarashi, I., Saito, A., Fujisaki, K., Mikaimi, T., Suzuki, N., Toyoda, Y.: Increased susceptibility to Toxoplasma gondii infection in SAG-1 transgenic mice. Int. J. parasitol. 29:1433-1436, 1999.

238. Nishikawa, Y., Xuan, X., Kimura, M., Otsuka, H.: Characterization of pseudorabies virus glycoprotein B expressed by canine herpesvirus. J. Vet. Med. Sci. 61:1113-1117, 1999.

239. Tanaka, T., Xuan, X., Igarashi, I., Nagasawa, H., Fujisaki, K., Suzuki. and Mikami, T.: Detection of antibodies to Babesia equi by the latex agglutination test with recombinant merozoite antigen-2. J. Protozool. Res. 9:152-156, 1999.

240. Ikadai, H., Tamaki, Y., Xuan, X., Igarashi, I., Kawai, S., Nagasawa, H., Fujisaki, K., Toyoda, Y., Suzuki, N., Mikami, T.: Inhibitory effect of monoclonal antibodies on the growth of Babesia caballi. Int. J. Parasitol. 29:1803-1808, 1999.

241. Tanaka, T., Xuan, X., Ikadai, H., Igarashi, I., Fujisaki, K., Mikami, T., Suzuki, N.: Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA. Int. J. Parasitol. 29:1803-1808, 1999.

242. Ikadai, H., Xuan, X., Igarashi, I., Tanaka, S., Kanemaru, T., Nagasawa, H., Fujisaki, K., Suzuki, N., Mikami, T.: Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry and potential use of recombinant antigen in enzyme-linked immunosorbent assay. J. Clin. Microbiol. 37:3475-3480, 1999.

243. Tanaka, T., Xuan, X., Kato, M., Nagasawa, H., Fujisaki, K., Mikami, T., Suzuki, N.: Expression of recombinant Toxoplasma gondii P24. J. Vet. Med. Sci. 61:1235-1239, 1999.

1998

244. Xuan, X., Nishikawa, Y., Takashima, Y., Tuchiya, K., Ueda, S., Yokoyama, N., Maeda, K., Mikami, T., Otsuka, H.: Construction of canine herpesvirus vector expressing foreign genes using a lacZ-TK gene cassette as a double selectional marker. Virus Genes 17:25-32, 1998.

245. Inoue,N., Honzako,, Y., Hirumi, K., Xuan, X., Nagasawa, H., Mikami, T., Hirumi, H.: Kinetoplast DNA and procyclic acidic repetitive protein A-α gene of Trypanosoma evansi. J. Protozool. Res. 8:28-43, 1998.

246. Xuan. X., Igarashi, I., Ikadai, H., Inoue, N., Nagasawa, H., Fujisaki, K., Toyoda, Y., Suzuki, N., Mikami, T.: Diagnosis of Babesia caballi infection in horses by polymerase chain reaction. J. Protozool. Res. 8:85-89, 1998.

247. Xuan, X., Tuchiya, K., Sato, I., Nishikawa, Y., Takashima, Y., Yamamoto, A., Ueda, S., Mikami, T., Otsuka, H.: Biological and immunogenic properties of rabies virus glycoprotein expressed by canine herpesvirus vector. Vaccine 16:969-976, 1998.

248. Nishikawa, Y., Xuan, X., Otsuka, H.: Identification and characterization of the glycoprotein E and I genes of canine herpesvirus. Virus Res. 56:77-92, 1998.

1997

249. Xuan, X., Kojama, A., Murata, T., Mikami, T., Otsuka, H.: Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus. Arch. Virol. 142:1003-1010, 1997.

250. Yoshitake, N., Xuan, X., Otsuka, H.: Identification and characterization of bovine herpesvirus-1 glycoproteins E and I. J. Gen. Virol. 78:1399-1403, 1997.

251. Maeda, K., Xuan, X., Kawaguchi, Y., Ono, M., Yokoyama, N., Fujita, K., Tohya, Y., Mikami, T.: Characterization of canine herpesvirus glycoprotein D (hemagglutinin). J. Vet. Med. Sci. 59:1003-1009, 1997.

252. Maeda, K., Yokoyama, N., Fujita, K., Xuan, X., Mikami, T.: Role of one N-linked oligosaccharide chain on canine herpesvirus gD in its biological activity. J. Vet. Med. Sci. 12:1123-1128, 1997.

1996

253. Otsuka, H., Xuan, X.: Construction of bovine herpesvirus-1 (BHV-1) recombinants which express pseudorabies virus (PRV) glycoproteins gB, gC, gD, and gE. Arch. Virol. 141:57-71, 1996.

254. Xuan, X., Maeda, K., Tohya, Y., Mikami, T., Otsuka, H.: Identification and nucleotide sequence of the thymidine kinase gene of canine herpesvirus. Virus Genes 12:185-188, 1996.

255. Otsuka, H., Xuan, X., Shibata, I., Mori, M.: Protective immunity of bovine herpesvirus-1 (BHV-1) recombinants which express pseudorabies virus (PRV) glycoproteins gB, gC, gD and gE. J. Vet. Med. Sci. 58:819-824, 1996.

256. Xuan, X., Maeda,K., Mikami, T. and Otsuka, H.: Characterization of CHV glycoprotein C expressed by a recombinants baculovirus in insect cells. Virus Res. 46:57-64, 1996.

1995

257. Xuan, X., Nakamura, T., Ihara, T., Sato, I., Tuchiya, K., Nosetto, E., Ishihama, A., Ueda, S.: Characterization of pseudorabies virus glycoprotein gII expressed by recombinant baculovirus. Virus Res. 36:151-161, 1995.

1992

258. Xuan, X., Horimoto, T., Limcumpao, J. A., Tohya, Y., Takahashi, T., Mikami, T.: Glycoprotein-specific immune response in canine herpesvirus infection. Arch. Virol. 122:359-365, 1992.

259. Horimoto, T., Limcumpao, J. A., Xuan, X., Ono, M., Maeda, K., Kawaguchi, Y., Kai, C., Takahashi, E., Mikami, T.: Heterogeneity of feline herpesvirus type 1 strains. Arch. Virol. 126:283-292, 1992.

1991

260. Xuan, X., Horimoto, T., Limcumpao, J. A., Takumi, A., Tohya, Y., Azetaka, M., Takahashi, E., Mikami, T.: Neutralizing determinants of canine herpesvirus as defined by monoclonal antibodies. Arch. Virol. 116:185-195, 1991.

261. Limcumpao, J. A., Horimoto, T., Xuan, X., Tohya, Y., Azetaka, M., Takahashi, E., Mikami, T.: Homologous and heterologous antibody responses of mice immunized with purified feline herpesvirus type 1 and canine herpesvirus glycoprotein. J. Vet. Med. Sci. 53:423-432, 1991.

262. Limcumpao, J. A., Xuan, X., Horimoto, T., Tohya, Y., Azetaka, M., Takahashi, E., Mikami, T.: Intracellular localization and transport of canine herpesvirus glycoproteins. J. Vet. Med. Sci. 53:517-519, 1991.

263. Xuan, X., Limcumpao, J. A., Horimoto, T., Tohya, Y., Azetaka, M., Takahashi, E., Mikami, T.: Induction of antibodies to canine herpesvirus in mice by immunization with anti-idiotypic antibodies. Vet. Microbiol. 28:257-267, 1991.

1990

264. Takumi, A., Kusanagi, K., Tuchiya, K., Xuan, X., Azetaka, M., Takahashi, E.: Serodiagnosis of canine herpesvirus infection: development of an enzyme-linked immunosorbent assay and its comparison with two improved methods of serum neutralization test. Jpn. J. Vet. Sci. 52:241-250, 1990.

265. Nemoto, K., Horimoto, T., Xuan, X., Kusanagi, K., Takumi, A., Tohya, Y., Azetaka, M., Takahahsi, E., Mikami, T.: Demonstration of canine herpesvirus-specific hemagglutination. Jpn. J. Vet. Sci. 52:395-398, 1990.

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