Potato Germplasm Enhancement Laboratory

Obihiro Univerisity of Agriculture and Veterinary Medicine
West 2-11, Inada, Obihiro, Hokkaido 080-8555, Japan

How to make aceto-carmine

  1. Add 2.5 g of carmine (Carmine "CertistainR", Merck) into 250 ml of 45% acetic acid in a 500 ml Erlenmeyer flask.

  2. Assemble a cooling tower on the flask as shown below.

  3. Keep boiling for 48 hours.

  4. After cooling down, filtrate through a filter paper.

  5. Store in a refrigerator. (Older is better)