Potato Germplasm Enhancement Laboratory

Obihiro Univerisity of Agriculture and Veterinary Medicine
West 2-11, Inada, Obihiro, Hokkaido 080-8555, Japan

Pollen fertility test by acetocarmine stainability

  1. Collect at least 3 mature flowers per plant.

  2. Place one drop of 1% acetocarmine on a slide glass.

  3. Put the pollen slightly on the acetocarmine drop using a vibrator (an electric toothbrush without a brush part is convenient).

  4. Leave for 1 minute, and then gently place a cover slip.

  5. Observe al least 200 pollen grains under a microscope. Count the numbers of well-stained pollen grains and unstained, aborted pollen grains.

Note: Pollen stainability less than 5% is classified as male sterile. Plant which has more than 1% of 2n pollen is considered as a 2n pollen producer.